I am working on a project for cell segmentation.
After edge detection using canny operator , I get the image like this.
Nuclei of the cell is given by innermost circle and the circular shape surrrounding it is the cytoplasm.
Now I need to get the closed boundary for cytoplasm, so that I can fill the cell body and use it as mask for segmentation.
Please guide me on the matlab functions that can be used for closing the circular shape containing nuclei.