image segmentation using matlab GUI

hi i m doing a project on medical image authentication through watermarking. i need to segment the region of interest and region of non interest of the medical image as follows Steps in ROI separation approach

1. Mouse click function: For selecting the ROI, mouse clicking function is used.
2. Done button: To get the output after selection process, done button is implemented.
3. Storing handles: For safe storing the pixels values of selected ROI (Xmin,Xmax, Yminand Ymax) and image of selected ROI, the storing handles are used.
4. Start button: It is implemented to clear the stored handles to start again the process ofselecting ROI.
5. Zooming option: It is provided for zooming the image, so that the image will be clear toselect the ROI.

please guide me how to segment the same

Answer 1

[Image Analyst]

These links will help you:

http://www.mathworks.com/matlabcentral/fileexchange/24224-magic-matlab-generic-imaging-component

http://www.mathworks.com/matlabcentral/fileexchange/25157-image-segmentation-tutorial

Also see the attached demos on zooming and masking.