HOW TO CONVERT PIXEL VALUES TO MICROMETER

CAN SOME ONE PROVIDE ME WITH THE FOLLOWING INFORMATION. IAM WORKING ON A FINAL PROJECT.

HOW TO CONVERT PIXEL VALUES TO MICROMETER (FORMULA)

HOW TO MAGNIFY AND CAPTURE A IMAGE TO MEASURE ITS VALUES IN MICROMETERS.

WHAT IS THE DESIGN FACTORS TO BE CONSIDERED (FORMULA) TO OBTAIN THE SAME

Answer 1

[Image Analyst]

YOU NEED TO IMAGE AN OBJECT OF A KNOWN LENGTH, SUCH AS A RULER OR PRECISION SPHERES.

[Image Analyst]

See my attached spatial calibration demo.

[Devyani Sankhe]

How do you calculate for this image, can you please tell me an code ? here is the my image attached, Please do let me know the matlab code to calculate further dimension of any other images. Thank you so much

[Image Analyst]

Devesh, I did tell you and did give you the code. Why didn't you run my attached demo and draw a line across your micron bar on the bottom to calibrate, and then draw the vertical distance to get the height of the object?

Or to get automatically, do your spatial calibration and then threshold the image and get the bounding box, and then multiply the bounding box height in pixels by your micronsPerPixel calibration factor. See my Image Segmentation Tutorial in my File Exchange for a full demo:

Image Analyst's File Exchange

Answer 2

[Alaa]

Actually, I need same code , is anyone can help us?