Detecting nuclei from a microscopic image

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Sam Lee el 7 de Abr. de 2018

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https://la.mathworks.com/matlabcentral/answers/393345-detecting-nuclei-from-a-microscopic-image

Cerrada: John D'Errico el 7 de Abr. de 2018

I have these microscopic images from which I need to detect the nuclei. I sharpened the image using "localcontrast()" and the nuclei look pretty decent. After, I tried reducing noise using medfilt3() but most of the nuclei disappeared. How can I solve this problem?

Here is one of the images after sharpening (need to keep only the green spots):