Clearing background of microscopy images

Hello Everyone,

I have to measure the size of the blobs in the example image, but I cannot threshold them because some parts of the scratches have similar colors, so either I have to reduce the size of the blobs while thresholding, or I end up with a lot of smaller blobs from the scratches that end up skewing my distribution (like the second image). I have tried blurring the images and thresholding individual color channels (the scratches have higher average red values), but couldn't get a passable result without losing a lot of my actual features. Can someone point me towards a method/function that can help?

Thanks!

Answer 1

[Seyeong Jeon]

I recommend you to add this line below your example result code.

image = imclose(image, ones(5));