error in cell segmentation

i want to make this overlapping cell segmentation same as this link. but when i using their coding in my coding they not work. they have some error. but i dont know how to solve it. please see the link and my coding in pdf

http://blogs.mathworks.com/steve/2006/06/02/cell-segmentation/

[izyan hanum]

this is my coding

[Image Analyst]

There are so many line breaks introduced by the PDF that it would take long than I'm willing to spend for you to fix them. Please attach the m-file itself.

[izyan hanum]

clc;

close all;

clear;

workspace;

format long g;

format compact;

fontSize = 14;

figure;

set(gcf, 'Renderer', 'Painters');

folder = 'C:\Users\User\Pictures';

baseFileName = 'N8.jpg';

fullFileName = fullfile(folder, baseFileName);

if ~exist(fullFileName, 'file')

    fullFileName = baseFileName; 
    if ~exist(fullFileName, 'file')

        errorMessage = sprintf('Error: %s does not exist.', fullFileName);
        uiwait(warndlg(errorMessage));
        return;
    end
end
rgbImage = imread(fullFileName);
[rows, columns, numberOfColorBands] = size(rgbImage);
figure(1),subplot(3, 3, 2);
imshow(rgbImage);
title('Original Sputum Image', 'FontSize', fontSize);
set(gcf, 'Units', 'Normalized', 'Outerposition', [0, 0, 1, 1]);

hsv = rgb2hsv(rgbImage);

hImage = hsv(:, :, 1);

sImage = hsv(:, :, 2);

vImage = hsv(:, :, 3);

figure(1),subplot(3, 3, 4);

imshow(hImage, []);

title('Hue Image', 'FontSize', fontSize);

figure(1),subplot(3, 3, 5);

imshow(sImage, []);

title('Saturation Image', 'FontSize', fontSize);

figure(1),subplot(3, 3, 6);

imshow(vImage, []);

title('Value Image', 'FontSize', fontSize);

hueBinary = hImage > 0.2 | hImage < 0.1;

saturationBinary = sImage > 0.22;

valueBinary = vImage > 0.28 & vImage < 0.9;

binaryImage = hueBinary & saturationBinary & valueBinary;

binaryImage = bwareaopen(binaryImage, 400);

binaryImage = imfill(binaryImage, 'holes');

figure(2),subplot(2, 2, 1);

imshow(binaryImage, []);

axis on;

title('Opening', 'FontSize', fontSize);

se = strel('disk',10);

afterclosing = imclose(binaryImage,se);

figure(2), subplot (2,2,2), imshow(afterclosing,[]);

axis on;

title('Closing','FontSize', fontSize);

set(gcf, 'Units', 'Normalized', 'Outerposition', [0, 0, 1, 1]);

bw2 = imfill(afterclosing,'holes');

bw3 = imopen(bw2, ones(5,5));

bw4 = bwareaopen(bw3, 40);

bw4_perim = bwperim(bw4);

overlay1 = imoverlay(sImage, bw4_perim, [.3 1 .3]);

imshow(overlay1);

mask_em = imextendedmax(sImage, 30);

imshow(mask_em);

mask_em = imclose(mask_em, ones(5,5));

mask_em = imfill(mask_em, 'holes');

mask_em = bwareaopen(mask_em, 40);

overlay2 = imoverlay(sImage, bw4_perim | mask_em, [.3 1 .3]);

imshow(overlay2);

I_eq_c = imcomplement(I_eq);

I_mod = imimposemin(I_eq_c, ~bw4 | mask_em);

L = watershed(I_mod);

figure(3),imshow(label2rgb(L));

[izyan hanum]

sir i already attach my coding i know the error at overlay

[Image Analyst]

I don't have time left today. In the meantime, read this: http://www.mathworks.com/matlabcentral/answers/13205-tutorial-how-to-format-your-question-with-markup

[izyan hanum]

      clc;

      close all;

      clear;

      workspace;

      format long g;

      format compact;

      fontSize = 14;

      figure;

      set(gcf, 'Renderer', 'Painters');

      folder = 'C:\Users\User\Pictures';

      baseFileName = 'N8.jpg';

      fullFileName = fullfile(folder, baseFileName);

      if ~exist(fullFileName, 'file')

          fullFileName = baseFileName; 
          if ~exist(fullFileName, 'file')

              errorMessage = sprintf('Error: %s does not exist.', fullFileName);
              uiwait(warndlg(errorMessage));
              return;
          end
      end
      rgbImage = imread(fullFileName);
      [rows, columns, numberOfColorBands] = size(rgbImage);
      figure(1),subplot(3, 3, 2);
      imshow(rgbImage);
      title('Original Sputum Image', 'FontSize', fontSize);
      set(gcf, 'Units', 'Normalized', 'Outerposition', [0, 0, 1, 1]);

      hsv = rgb2hsv(rgbImage);

      hImage = hsv(:, :, 1);

      sImage = hsv(:, :, 2);

      vImage = hsv(:, :, 3);

      figure(1),subplot(3, 3, 4);

      imshow(hImage, []);

      title('Hue Image', 'FontSize', fontSize);

      figure(1),subplot(3, 3, 5);

      imshow(sImage, []);

      title('Saturation Image', 'FontSize', fontSize);

      figure(1),subplot(3, 3, 6);

      imshow(vImage, []);

      title('Value Image', 'FontSize', fontSize);

      hueBinary = hImage > 0.2 | hImage < 0.1;

      saturationBinary = sImage > 0.22;

      valueBinary = vImage > 0.28 & vImage < 0.9;

      binaryImage = hueBinary & saturationBinary & valueBinary;

      binaryImage = bwareaopen(binaryImage, 400);

      binaryImage = imfill(binaryImage, 'holes');

      figure(2),subplot(2, 2, 1);

      imshow(binaryImage, []);

      axis on;

      title('Opening', 'FontSize', fontSize);

      se = strel('disk',10);

      afterclosing = imclose(binaryImage,se);

      figure(2), subplot (2,2,2), imshow(afterclosing,[]);

      axis on;

      title('Closing','FontSize', fontSize);

      set(gcf, 'Units', 'Normalized', 'Outerposition', [0, 0, 1, 1]);

      bw2 = imfill(afterclosing,'holes');

      bw3 = imopen(bw2, ones(5,5));

      bw4 = bwareaopen(bw3, 40);

      bw4_perim = bwperim(bw4);

      overlay1 = imoverlay(sImage, bw4_perim, [.3 1 .3]);

      imshow(overlay1);

      mask_em = imextendedmax(sImage, 30);

      imshow(mask_em);

      mask_em = imclose(mask_em, ones(5,5));

      mask_em = imfill(mask_em, 'holes');

      mask_em = bwareaopen(mask_em, 40);

      overlay2 = imoverlay(sImage, bw4_perim | mask_em, [.3 1 .3]);

      imshow(overlay2);

      I_eq_c = imcomplement(I_eq);

      I_mod = imimposemin(I_eq_c, ~bw4 | mask_em);

      L = watershed(I_mod);

      figure(3),imshow(label2rgb(L));

[izyan hanum]

done sir. thank you for helping me to send .m file here. but please see my error. i want to make as link given but they have error at overlay