Counting wheat grains and marking them with image processing

I cannot seperate grains in groups properly, and it makes counting and marking not good.

[John D'Errico]

If you want help, then show what you tried, at least say what you tried. Otherwise, you become no different from the hordes of students who make no effort, and just demand someone do their homework for them.

[Robert]

Thanks, i forgot to attach the matlab code, that i wrote for this. I think the counting is working within 5% of error, but i have problems with separating the grains in huge clusters properly, thats why i cannot mark them well.

[Image Analyst]

@Robert did you even try the suggestions I gave you in my answer below (scroll down)? If not, why not?

Answer 1

[Image Analyst]

First of all, you need to improve your image capture setup. Why do you have such a horrible, colored, and non-uniform lighting? That should be easy to fix. Secondly you will need to do a background correction by dividing the test image by the background. MATLAB also has a background flattening function in one of its toolboxes. Then you can segment the image.

See my Image Segmentation Tutorial in my File Exchange:

Image Analyst's File Exchange

It's a generic, general purpose demo of how to threshold an image to find blobs, and then measure things about the blobs, and extract certain blobs based on their areas or diameters.

As you said, some grains are touching so you'll need to split them apart. See Steve's blogs on how to do this:

Watershed transform question from tech support » Watershed transform question from tech support

Marker-Controlled Watershed Segmentation - MATLAB & Simulink Example