Process some SEM pictures of electeospun fibrous materials through programming of Matlab, and the aiming result is getting fibre diameter.

I'm planning to process some SEM pictures of electeospun fibrous materials through Matlab, and the aiming result is getting fibre diameter. Once I input the picture, the program or model will directly output the fibrous diameter, including distribution, average value, ect. Where should I start? Looking forward to your reply :)

Answer 1

[Umeshraja]

Hi @Yuzhuo,

I understand you're looking to analyze electrospun fiber diameters from SEM images using MATLAB. One useful tool I found for this is available on MATLAB File Exchange: Fiber Diameter Distribution. This tool requires spatial resolution input and generates Excel files with diameter statistics.

Another option is this fiber detection and measurement tool, which also helps in diameter analysis but is optimized specifically for 1024x768 resolution images

You might also consider SIMPoly, a MATLAB-based semi-automated tool with a user-friendly GUI. It's quick and easy to use, and includes features like histogram fitting and color mapping. This tool is described in the following publication:

Murphy R, Turcott A, Banuelos L, et al. SIMPoly: A Matlab-Based Image Analysis Tool to Measure Electrospun Polymer Scaffold Fiber Diameter. Tissue Eng Part C Methods. 2020 Dec;26(12):628–636.DOI: 10.1089/ten.TEC.2020.0304

Additionally, for a deeper understanding of MATLAB algorithms used in fiber diameter measurements—particularly those involving image processing techniques like the Circular Hough Transform—you may find this reference helpful:

Abdelkader M. MATLAB Algorithms for Diameter Measurements of Textile Yarns and Fibers through Image Processing Techniques. Materials (Basel). 2022 Feb 10;15(4):1299. doi: 10.3390/ma15041299. PMID: 35207840; PMCID: PMC8875885.

Hope this helps!