Why bwareaopen() is not working for my code?

Dear all,
I have this code, where I want to segmentation larger blobs (for example bigger than 1 pixel) for following work. I use function bwareaopen for this, but in output, there is only one blob segmented. I don´t know, where is mistake.
I attach code and original image. Thank you for your answers.

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el 6 de Mzo. de 2017

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