saving coloured image in gui

hiii all!!' i was trying to save an image in tif format after doing maximum intensity projection!!whenever m saving the image stored is in grey scale...my original image colour is green....can anyone give me a solution!!thanks in advance

[Geoff Hayes]

ash - please show the code that you have written to save the image.

[ash fairy]

save=newfile('*.tif','SAVE FILE');

 imwrite(new,save,'WriteMode','append');

Answer 1

[Image Analyst]

Are you SURE? Have you passed the image you're passing to imwrite() into the size() function and seen what it says?

[rows, columns, numberOfColorChannels] = size(yourImage) % No semicolon
whos yourImage

What gets reported to the command line for numberOfColorChannels and the data type? If it's 1 instead of 3, then it's gray scale, NOT color. If it's 3, it should save as color, if it's uint8.

[Image Analyst]

Regarding your code:

save=newfile('*.tif','SAVE FILE');
imwrite(new,save,'WriteMode','append');

Do not use save as the name of your variable. It is the name of an important build in function, which you just (temporarily) destroyed.

Also, please answer my original questions!

[ash fairy]

hi, this is my original code axes(handles.axes1); myimage=getimage; saveeeee=uiputfile('*.tif','SAVe'); A=get(hObject,'Value') if A==1 z = zeros(256, 1); ramP = (0:255)' / 255; cmaP = [z, ramP, z];

        myimage=ind2rgb(im2uint8(myimage),colormap(cmaP));
        imwrite(myimage,saveeeee)

now the file gets saved as green color , but the thing is i want to save my file as 16bit green color(im2uint16 did not work right). currently when i save the file its rgb file. my goal is to get a 16bit color image so that i can read it into imagej

[Image Analyst]

Ash fairy, you need to make a full size colormap, then you need to do this:

rgbImage = ind2rgb(imageInAxes, colormap(cmap));
rgbImage16 = im2uint16(rgbImage);

See this full demo code:

% Initialization
gray8 = imread('cameraman.tif');
gray16 = im2uint16(gray8);
subplot(1, 3, 1);
hAxis = imshow(gray16);
whos gray16

% OK, let's start.
imageInAxes = getimage(hAxis);
whos imageInAxes % Should be uint16.

% Create green color map.
maxGL = double(intmax(class(gray16)))
z = zeros(maxGL + 1, 1);
ramp = (0 : maxGL)' / maxGL;
cmap = [z, ramp, z];

% Convert gray scale 16 bit image to color using colormap
rgbImage = ind2rgb(imageInAxes, colormap(cmap));
whos rgbImage
% Right now rgbImage is double in the range 0-1.

% Convert to uint16 in the range 0-65535
rgbImage16 = im2uint16(rgbImage);
subplot(1, 3, 2);
imshow(rgbImage16);
whos rgbImage

% Ask user to save the image.
[baseFileName, folder] = uiputfile('*.tif','Save the image?');
fullFileName = fullfile(folder, baseFileName)
imwrite(rgbImage16, fullFileName)

% Recall it from disk to check that it looks normal
recalledImage = imread(fullFileName);
subplot(1, 3, 3);
imshow(recalledImage);