How can the intensity of each cell be measured?

Hello,

I have an image dataset like the example below. In each image, two different types of cells are intertwined. There are more intracellular elements in one cell type than in another. This makes the intensity of the cell higher. So you can look at the intensity of each cell to classify the cells. The red and green dots in the current image show the center point of different cells. This image has been processed by another team and the cells have been detected. I will use images in which these points are not added at all.

However, I am not sure how to do this. So what can be done as a method, I have a lack in this regard. For example, should I make each cell into clusters? Is it possible to measure the intensity of each cluster?

You can answer it from both Matlab at the code level and with logical explanations.

Thank you from now

Answer 1

[Rahul]

Hi @Beyza Taskin,

In order to find the intensity of each pixel of the image, functions like 'mean' or 'find' functions while defining thresholds for finding the desired intensity pixels. Examples can be seen in the following MATLAB Answers:

https://www.mathworks.com/matlabcentral/answers/40195-image-intensity

https://www.mathworks.com/matlabcentral/answers/86484-how-to-find-the-intensity-of-each-pixel-of-an-image

Additionaly, 'improfile' function can be used to obtain an Intensity profile of the image by either defining the pixel region manually or drawing it on the figure. Here is an example:

imshow("your_image.png")
improfile
% Here the region to detect intensity values can be drawn on the shown figure

Refer to the following MathWorks documentations to knwo more:

'mean': https://www.mathworks.com/help/releases/R2021a/matlab/ref/mean.html

'find': https://www.mathworks.com/help/releases/R2021a/matlab/ref/find.html

'improfile': https://www.mathworks.com/help/releases/R2021a/images/ref/improfile.html

Thanks.